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Department of Oncology


My research combines developmental neurobiology, epigenetics, stem cell science and genomics approaches to reveal the role of chromatin remodelling in paediatric high-grade glioma initiation, maintenance and therapy resistance.

​Specific techniques we employ include:

  • De novo glioma model development using in utero electroporation of transposons and CRISPR vectors 
  • Transgenics
  • In vivo imaging (bioluminescence)
  • Tissue histology and immunofluorescence (cryostat/vibratome sectioning, confocal and epifluorescence microscopy)
  • Single-cell dissociation of fixed and unfixed tissue
  • Neural stem cell work (stem cell culture, stereotactic injection, quiescence/proliferation/differentiation analysis following transfection in vitro and in vivo)
  • Flow cytometry (FACS)
  • Genomics (RNA-seq/ChIP-seq)
  • Molecular biology (cloning, transposon and viral vector design)
  • Biochemistry (Western blotting, chromatin immunoprecipitation, acid extraction of histones)

For further information about our research, neural stem cells, chromatin remodelling and paediatric high-grade gliomas see the Pathania Lab website.


Key publications: 

Pathania, M. et al. (2017) H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas. Cancer Cell 32 (5), 684-700 e9.

Research Group Leader, CRUK Cambridge Centre Neuro-Oncology & Onco-Innovation Programmes
Portrait photo of Manav Pathania

Contact Details

Department of Oncology
University of Cambridge
The Milner Therapeutics Institute
Jeffrey Cheah Biomedical Centre
Puddicombe Way
Cambridge Biomedical Campus
Takes PhD students
Not available for consultancy