skip to content

Department of Oncology

 

My research combines developmental neurobiology, epigenetics, stem cell science and genomics approaches to reveal the role of chromatin remodelling in paediatric high-grade glioma initiation, maintenance and therapy resistance.

​Specific techniques we employ include:

  • De novo glioma model development using in utero electroporation of transposons and CRISPR vectors 
  • Transgenics
  • In vivo imaging (bioluminescence)
  • Tissue histology and immunofluorescence (cryostat/vibratome sectioning, confocal and epifluorescence microscopy)
  • Single-cell dissociation of fixed and unfixed tissue
  • Neural stem cell work (stem cell culture, stereotactic injection, quiescence/proliferation/differentiation analysis following transfection in vitro and in vivo)
  • Flow cytometry (FACS)
  • Genomics (RNA-seq/ChIP-seq)
  • Molecular biology (cloning, transposon and viral vector design)
  • Biochemistry (Western blotting, chromatin immunoprecipitation, acid extraction of histones)

For further information about our research, neural stem cells, chromatin remodelling and paediatric high-grade gliomas see the Pathania Lab website.

Publications

Key publications: 

Pathania, M. et al. (2017) H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas. Cancer Cell 32 (5), 684-700 e9. https://www.ncbi.nlm.nih.gov/pubmed/29107533

Research Group Leader, CRUK Cambridge Centre Neuro-Oncology & Onco-Innovation Programmes
Portrait photo of Manav Pathania

Contact Details

Department of Oncology
University of Cambridge
Clifford Allbutt Building
Cambridge Biomedical Campus
Cambridge
CB2 0AH
Takes PhD students